Table 1. Gene-specific primer pairs used to characterise the different AhMTP1 paralogues in real-time quantitative RT–PCR analyses.
Gene | Primer name | Primer sequence 5′--3′ | Annealing temperature (°C) | Average PCR efficiencies | |
gDNA | cDNA | ||||
Actin | ActinF | GGTAACATTGTGCTCAGTGGTGG | 65.9 | 1.87 | 1.90 |
ActinR | AACGACCTTAATCTTCATGCTGC | ||||
AhMTP1-A1 &A2 | ARTF | AGTGGTGAACATCATAATGGCTGTTC | 67.2 | 1.96 | 1.93 |
ACRTR | TCGATTTGTCCAACAGTTGCTCAG | ||||
AhMTP1-B | BRTF | TGGACGTGAAGTTATGGAGAC | 64.3 | 2.06 | 2.04 |
BRTR | CTTCACATTTGGGCTATCACAG | ||||
AhMTP1-C | CRTF | GGTGAACATCATAATGGCTGTTATGCTG | 67.8 | 1.98 | NP a |
ACRTR | TCGATTTGTCCAACAGTTGCTCAG | ||||
AhMTP1-D | DRTF | GTACACCCAGAGAGATTGACGCCG | 71.3 | 1.91 | NP a |
DRTR | GACTAATGTTGTACTCCCTGCGGATG |
a NP: Not possible to be done (see Materials and Methods).