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. 2010 Apr 15;6(4):e1000842. doi: 10.1371/journal.ppat.1000842

Figure 6. Histopathologic studies showed evidence of BrdU+ cells, the majority of which are Mac387+, in the brains of macaques with SIVE.

Figure 6

A–B: Immunohistochemistry with an antibody against BrdU is utilized in brain sections of rapid progressors. A: BrdU+ cells are present in and around SIVE lesions (BrdU: DAB, brown) B: BrdU+ cells are seen in and around the vasculature (BrdU: DAB, brown) C: Immunohistochemistry with antibodies against CD3 (Vector Blue, blue) and BrdU (DAB, brown) is utilized in brain sections of rapid progressors to examine if BrdU+ cells are CD4+ T lymphocytes. No double label BrdU+ and CD3+ cells are found in the brains of SIVE+ macaques. D and E: Immunohistochemistry with antibodies against GFAP (Vector Blue, blue) and BrdU (DAB, brown) is utilized in brain sections of rapid progressors to examine if BrdU+ cells are astrocytes. There are very few BrdU+ astrocytes seen in all sections examined. The arrow points to a BrdU+ in a vessel surrounded by astrocyte foot processes. The asterisk points out a BrdU+ cells in close proximity to GFAP+ astrocytes. F and G: Immunohistochemistry with antibodies against CD68 (DAB, brown) and BrdU (Vector Blue, blue) is utilized in brain sections of rapid progressors to examine if BrdU+ cells were CD68+ mature macrophages. Few BrdU+CD68+ cells are seen. H and I: Double staining with antibodies against BrdU (Vector Blue, blue) and Mac387 (DAB, brown) is utilized to determine if BrdU+ cells in the brain are early monocyte/macrophage infiltrates. BrdU and Mac387 did co-localization in the brain of SIVE animals. The arrows indicate BrdU+Mac387+ monocyte/macrophages, in a lesion (H) or perivascular region (I). The asterisk is a Mac387 BrdU- cell in the vasculature. Brain sections are representative of three SIVE+ animals examined for all stains.