Figure 2. Immunoblot analysis of velocity sedimented PrP material from tg338 mouse brain.

Brain homogenates from uninfected (A) or scrapie-infected (B–C; LA21K strain) tg338 mice were solubilized and fractionated by sedimentation velocity. The collected fractions (numbered from top to bottom of the gradient) were analyzed for PrP content by immunoblot without (A, B) or after PK digestion (C). (D) Graph showing the relative amount of PrP^C (green line) and PK-resistant PrP^Sc (black line) per fraction. MM: molecular markers.