Figure 8. Role of PIP₃ formation during reversal of polarization.

Selected frames from Movies S6 just after insertion of the micropipette dispensing 300 nM UK 14'304 (0 sec, upper images) and after 5:40 min (lower images). The emission of THP-1 cells transduced with PH-PKB-mCherry (red fluorescence, 594 nm excitation/610–680 nm emission) and expressing α_2AAR-YFP/CFP (yellow fluorescence YFP, 514 nm excitation/525–580 nm emission) was recorded contemporaneously. The false color shows the ratio of red/yellow (right panels). Gray, corresponding phase image. (A) control cells, (B) cell pretreated for 10 min with 100 nM Wortmannin. (514 nm excitation/525–590 nm emission), (Phase) phase images taken with DIC settings, and (Ratio) False color shows the ratio of red/yellow.