FIG. 2.

KA induces CYP1A1/2 metabolism and differentially activates the human AHR relative to the mouse AHR in reporter cell lines. (A) CYP1A1/2 metabolic activity was measured by CEE luciferase in primary human hepatocytes treated with KA, TCDD, or DMSO for 18 h. Experiment was performed in triplicate; error bars denote SD. * or ***Significance at p < 0.05 or p < 0.001, as compared to respective control-treated samples. (B) HepG2 40/6 and (C) H1L1.1c2 cell lines were exposed to increasing concentrations of KA for 4 h. The EC₂₅ values are defined as the dose of KA that yields 25% of the luciferase activity obtained with 10nM TCDD.