FIG. 4.

KA and IL1B synergistically induce IL6 gene expression and requires AHR expression. (A and B) MCF-7 cells were treated with increasing doses of KA for 2 h with or without IL1B and subjected to quantitative real-time PCR for IL6 and CYP1A1. (C) MCF-7 cells were electroporated with control or AHR siRNA and analyzed 48 h later after a 3-h exposure to IL1B in the presence or absence of KA. Western blot analyses of whole-cell extract, AHR, and p23 protein levels were assessed. (C and D) Real-time PCR analysis was performed for IL6 and CYP1A1 following 2-h KA treatment 48 h after electroporation. Experiments were performed in triplicate; error bars denote SD. * or ***Significance at p < 0.05 or p < 0.001, as compared to respective control-treated samples. Statistical analysis in panel A was performed relative to their respective vehicle controls.