FIG. 5.

Liver acetaldehyde content is reduced by methyl donor–enriched diet following subchronic alcohol exposure. Activity (A) and protein content (B) for ALDH1 and 2 were assessed in mice administered a control HFD or MDHFD with or without alcohol for 4 weeks. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Tim23 were used as cytosol and mitochondrial loading controls, respectively. (C) Acetaldehyde content in liver. (D) Semi-log correlation plot of acetaldehyde content with total pathology score (Spearman r² = 0.71). Asterisks indicate statistically significant (*p < 0.05 and **p < 0.01) differences using one-way ANOVA followed by Tukey’s post hoc test.