Table 1. Primer and probe sequences used in the quantitative PCR assays developed in this study.

PCR Assay	PCR Conditions	Amplicon Size	Primer/Probe	Primer/Probe Sequence
Mobiluncus spp.	60°C annealing, 39 s	219 bp	805F_Mob	5′-CCACGCTGTAAACGTTGGGAA-3′
	72°C extension, 30 s		1023R_Mob_curt	5′-TGGCCCATCTCTGGAACCA-3′
			1024R_Mob_mul	5′-CCACACCATCTCTGGCATG-3′
			Mob_838-861	5′-FAM-ATGCTATCCTGTGTTTCTGCGCCGTAG-TAMRA-3′
Lactobacillus iners	55°C annealing, 39 s	76 bp	165F_Liners	5′-GATGCTAATACCGGATAAYAACAGAT-3′
	72°C extension, 30 s		241R_Liners	5′-CACCGCAGGTCCATCCAAGA-3′
			Liners_186-213	5′-FAM-TGCCTATCAACTGTTTAAAAGATGGTTCT-TAMRA-3′
Lactobacillus jensenii	61°C annealing, 30 s	69 bp	988_Ljens	5′-GTCTTGACATCCTTTGACCAC-3′
	72°C extension, 30 s		1057R_Ljens	5′-CATGCACCACCTGTCTCTTT-3′
			Ljens_1009-1028	5′-FAM-CTAAGAGATTAGGTTTTCCC-MGBNFQ-3′