Table 1.
Clinical isolate phenotypes as a function of isolate culture site
| Site of culture (n) | Swim-positive [n (%)] | Twitch-positive [n (%)] | Swarm-positive [n (%)]* | Biofilm [median (range)]† | T3SS-positive [n (%)]‡ |
|---|---|---|---|---|---|
| Respir. (136) | 121 (89) | 100 (74 ) | 75 (57 ) | 0.98 (0.17–10) | 77 (57 ) |
| Urine (65) | 54 (83 ) | 49 (75 ) | 41 (63 ) | 0.96 (0.14–5.4) | 31 (48 ) |
| Wound (22) | 19 (86 ) | 18 (81 ) | 16 (76 ) | 0.74 (0.26–1.7) | 9 (41 ) |
| Blood (14) | 14 (100) | 13 (93 ) | 11 (85 ) | 1.2 (0.4–2.2) | 11 (80 ) |
| Total (237) | 208 (88 ) | 180 (76 ) | 143 (63 ) | 0.96 (0.14–10) | 128 (54 ) |
*Isolates were considered positive for swarming if the measured swarming zone diameter was >10 % of that measured for the PAO1 control. Ten isolates failed to grow on swarming medium so n=227 for this column.
†Biofilm formation on an abiotic surface was measured in a static assay and normalized to a PAO1 control.
‡A strain was considered positive for T3SS if it secreted >15 % as much PopD as the PA103 control, as measured by ELISA.