(A) Histone H4 resolved by SDS-PAGE was proteolytically digested, and the indicated modified peptides were identified by mass spectrometry.
(B) Crystal structure of the nucleosome showing the full histone octamer (left) or the H3/H4 tetramer (right). Histone H4 K 91 is highlighted in pink. Structure was generated with MOLSCRIPT and RASTER 3D by using PDB code 1ID3.
(C) 10-fold serial dilutions of cells, containing the indicated allele of histone H4, were plated on synthetic complete plates (HC) ± the indicated DNA damaging agent.
(D) For the analysis of UV sensitivity, cells were plated on HC plates and then subjected to the indicated doses of UV radiation.
(E–H) The indicated histone H4 alleles were introduced into strains deleted for factors involved in the DNA damage checkpoint (E), NHEJ (F), recombinational repair (G), or chromatin assembly (H).