Fig. 4.

U0126 increases activated Akt (pAkt) in the presence or absence of NMDA. Cerebellar granule cells were exposed to medium alone, U0126 (10 μM) or to a neuroprotective concentration of NMDA (100 μM) for 3 hr. At the indicated time, lysates were prepared and the proteins were separated by gel electrophoresis followed by transfer to nitrocellulose. Levels of pAkt and total levels of Akt were determined. Top: NMDA or U0126 induced activation of Akt (pAkt). Bottom: The blot was stripped and re-probed with an antibody that recognizes phosphorylated and unphosphorylated forms of Akt to demonstrate that an equal amount of Akt proteins were present in each lane n= 6. ^*P < 0.001 vs. untreated.