Figure 5.

Immunostaining of PC12 cells cultured in different conditions. After 5 days in culture, phalloidin–TRITC was used to stain the fixed PC12 cells on the PPy films. The PC12 cells cultured (a) in NGF-containing media on the PPy-NSE₅₀ films and (b) on NGF-conjugated PPy-NSE₅₀ films extended neurites, while those cultured (c) without NGF either in solution or on the PPy-NSE₅₀ films and (d) on NGF-treated regular PPy films did not form as many neurites as (a) or (b). Scale bar, 50 μm.