Figure 7.

In vitro PC12 cell culture on NGF-immobilized PPy-NSE₅₀ films after applying an external electrical potential. NGF-PPy-NSE₅₀ films were stimulated with an electrical potential of −1.0 V (versus SCE) for 5 min, followed by washing and PC12 cell culture. Neurotrophic activity (percentage of neurite-bearing cells) of the stimulated NGF-PPy-NSE₅₀ was similar to the two positive controls, (i) freshly prepared unstimulated NGF-PPy-NSE₅₀ and (ii) PPy-NSE₅₀ with exogenous NGF (50 ng ml⁻¹ in medium), but significantly different from the negative control of PPy-NSE₅₀ without NGF (p<0.01). Experiments were performed in triplicate.