Table 1.
PCR primers for amplification of cagA, vacA, iceA and oipA sequences
| Region | Primer | Primer sequence (5'-3') | Size of PCR product (bp) | References |
|---|---|---|---|---|
| cagA | F1 | GATAACAGCCAAGCTTTTGAGG | 349 | [36] |
| B1 | CTGCAAAAGATTGTTTGGCAGA | |||
| vacA | ||||
| m1 | VA3-F | GGTCAAAATGCGGTCATGG | 290 | |
| VA3-R | CCATTGGTACCTGTAGAAAC | |||
| m2 | VA4-F | GGAGCCCCAGGAAACATTG | 352 | |
| VA4-R | CATAACTAGCGCCTTGCAC | |||
| s1/s2 | VA1-F | ATGGAAATACAACAAACACAC | 259/286 | [12] |
| VA1-R | CTGCTTGAATGCGCCAAAC | |||
| s1b | SS3-R | AGCGCCATACCGCAAGAG | 187 | |
| VA1-R | CTGCTTGAATGCGCCAAAC | |||
| s1a | SS1-R | GTCAGCATCACACCGCAAC | 190 | |
| VA1-R | CTGCTTGAATGCGCCAAAC | |||
| s2 | SS2-R | GCTAACACGCCAAATGATGC | 199 | |
| VA1-R | CTGCTTGAATGCGCCAAAC | |||
| iceA1 | iceA1-F | GTGTTTTTAACCAAAGTATC | 247 | [37] |
| iceA1-R | CTATAGCCASTYTCTTTGCA | |||
| iceA2 | iceA2-F | GTTGGGTATATCACAATTTAT | 229/334 | [37] |
| iceA2-R | TTRCCCTATTTTCTAGTAGGT | |||
| oipA | HPO638F | GTTTTTGATGCATGGGATTT | 401 | [38] |
| HPO638R | GTGCATCTCTTATGGCTTT |