The effect of parameter changes on the load-displacement relationship. (a) The effect of rate on the load-displacement response for insertion and removal of the 200 μm diameter flat punch probe in the cortex. Average load-displacement curves comparing the response of insertion at the three different insertion rates (nFastLoad=2, nFastUnload=3, nMedium=3, nSlow=3). (b) The effect of probe size on the load-displacement response for the insertion and removal of the flat punch probe. Average load-displacement curves comparing the response of the 100 and 200 μm diameter probes inserted into the cortex at a rate of 822 μm/s (n200Load=2, n200Unload=3, n100Load=6, n100Unload=3). (c) Average load-displacement curves comparing the response of the 200 μm diameter probe with a flat punch and sharpened tip into the cortex (nFlatLoad=2, nFlatUnload=3, nSharp=3). (d) Effect of dura and pia on the load-displacement response to probe insertion and removal. Representative load-displacement curves for the insertion of the 100 μm diameter flat punch probe into the cortex at insertion rates of 822 μm/s with and without the dura and pia present prior to insertion.