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. 2009 Dec 2;29(48):15266–15276. doi: 10.1523/JNEUROSCI.4650-09.2009

Figure 3.

Figure 3.

Nogo-22 and CSPG inhibitory substrates inhibit ROCKII−/− dorsal root ganglion neuronal outgrowth to a lesser extent than ROCKII+/+ neurons. A–J, ROCKII+/+ and ROCKII−/− DRGs were cultured without inhibitor (A, B, F, G), with Nogo-22 (C, D), and with CSPG (H, I) and visualized with anti-βIII tubulin staining. ROCKII+/+ DRG neurite outgrowth was significantly reduced compared with ROCKII−/− neurite outgrowth in the presence of Nogo-22 (E, asterisk). ROCKII+/+ and ROCKII−/− DRG neurite outgrowth was significantly reduced in the presence of CSPG (J, asterisk). However, the effects of CSPG signaling on neuronal outgrowth were less inhibitory for ROCKII−/− neurons than for ROCKII+/+ DRGs (J, asterisk). Addition of soluble Y-27632 negated the inhibitory effects of Nogo-22 on ROCKII+/+ DRG neurite outgrowth and, to a lesser extent, CSPGs on ROCKII+/+ and ROCKII−/− DRG neurite outgrowth (E, J). Scale bar: (in A) A–I, 50 μm. All data are mean ± SEM, one-way ANOVA, p < 0.05.