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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Immunol. 2010 Mar 1;184(7):3628–3638. doi: 10.4049/jimmunol.0903851

Figure 4.

Figure 4

Nontransgenic LPL−/− cells demonstrate in vitro motility defects, though the in vivo phenotype of nontransgenic LPL−/− mice varies from that of transgenic LPL−/− mice. (A) Peripheral T cells isolated from LN of non-transgenic LPL−/− mice do not migrate efficiently in transwell assays towards CCL19 (100 ng/ml). Data shown are the mean ± S.E.M of triplicate samples in an individual experiment; representative of 3 independent experiments, p values determined by unpaired t-test. (B) Number of total, CD4SP, and CD8SP thymocytes recovered from non-transgenic WT (grey circles, n = 6) and LPL−/− (filled circles, n = 6) mice. Each symbol represents the value from one mouse, data from 6 independent analyses, mean represented by bar. (C) Expression of CD4 and CD8 on thymocytes from non-transgenic WT and LPL−/− mice. (D) Expression of the maturation markers CD69, CD24 and CD62L on CD4SP and CD8SP thymocytes from non-transgenic LPL−/− (solid line) and WT (grey histogram) mice. (E) Number of total, CD4+ and CD8+ cells recovered from 4 lymph nodes of non-transgenic WT (grey circles, n = 7) and LPL−/− (filled circles, n = 7) mice. Each symbol represents the value from one mouse, mean represented by bar, data from 7 independent analyses. Flow cytometry in (C and D) represents at least 4 pairs of mice.