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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Immunol. 2010 Mar 1;184(7):3628–3638. doi: 10.4049/jimmunol.0903851

Figure 6.

Figure 6

Early CCR7 signaling and CCR7-mediated TCR costimulation are not dependent upon LPL. (A) Levels of GTP-Rac in CD4+ T cells from WT (grey circles) and LPL−/− (filled circles) mice incubated with or without CCR7 ligands for 15 s. Duplicate samples indicated with symbols and mean indicated by bars; representative of 2 independent experiments. (B) F-actin content of CD4+ cells isolated from peripheral LN of WT (grey circles) and LPL−/− (filled circles) mice stimulated with CCL19 (100 ng/ml) and of CD4SP thymocytes sorted by FACS from WT (grey circles) and LPL−/− (filled circles) mice stimulated with CCL19 and CCL21 (100 ng/ml each). Data normalized to unstimulated cells. Each symbol represents result from 1 of 2 independent experiments with mean indicated by bars. (C) Conjugate formation of CFSE-labeled CD4+ T cells and DDAO-labeled CH27 cells incubated ± cognate peptide and ± CCL19 + CCL21. Data shown as percentage of T cells that formed conjugates. (D) Percent of cells positive for CD69 following overnight stimulation with APCs ± cognate peptide and ± CCL21. (C, D) Symbols represent replicate samples within one experiment and bars represent means. Representative of at least 2 independent experiments.