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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Immunity. 2006 May;24(5):623–632. doi: 10.1016/j.immuni.2006.02.015

Figure 2. Dendritic Cells Are Required for Rapid Activation of Salmonella-Specific T Cells in GALT.

Figure 2

Wild-type (WT BMQ) and DTR (CD11c-DTR BMQ) BM chimeras were injected with a single dose of 4 ng/g bodyweight of DT and adoptively transferred with SM1 T cells 6–8 hr later. Mice were infected orally the following day with Salmonella, and PPs and MLNs were harvested 12 hr later.

(A and B) PPs were harvested prior to adoptive transfer and DCs identified as MHCII+ CD11c+ (gated cells). Plots show (A) PP MHCII+ cells from CD11c-DTR chimeras with and without DT treatment and (B) the mean number ± SD of DCs in the PPs of each group of mice.

(C and D) Plots show the mean percentage ± SD of CD69+ or CD25+ SM1 T cells in the (C) MLNs, and (D) PPs of uninfected or infected BM chimeras 12 hr after oral infection. SM1 and activation marker gates were set similarly to those shown in Figure 1A. These data are similar to three other separate experiments.