| Component | Amount |
| pSCV-3ZFGNN | 10 μg |
| XmaI (10 U/μL) | 1.5 μL |
| SpeI (10 U/μL) | 1.5 μL |
| 10× Buffer (NEB 4) | 5 μL |
| 10× BSA | 5 μL |
| Nuclease-free water | to 50 μL |
| Total | 50 μL |
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