| Component | Amount |
| Purified ZF1 | 2.5 μg |
| XhoI (20 U/μL) | 1.5 μL |
| SpeI (10 U/μL) | 1.5 μL |
| 10× Buffer (NEB 2) | 5 μL |
| 10× BSA | 5 μL |
| Nuclease-free water | to 50 μL |
| Total | 50 μL |
| Component | Amount |
| Purified ZF1 | 2.5 μg |
| XhoI (20 U/μL) | 1.5 μL |
| SpeI (10 U/μL) | 1.5 μL |
| 10× Buffer (NEB 2) | 5 μL |
| 10× BSA | 5 μL |
| Nuclease-free water | to 50 μL |
| Total | 50 μL |