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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Protoc. 2010 Apr 1;5(4):791–810. doi: 10.1038/nprot.2010.34
Component Ligation Control
ZF1-2-3 vector 100 ng 100 ng
ZF4-5-6 insert 50 ng -------
5× T4 DNA Ligase Buffer 4 μL 4 μL
T4 DNA Ligase 1 μL 1 μL
Nuclease-free water to 20 μL to 20 μL
Total 20 μL 20 μL