| Component | Library | Clone |
| pSCV-Library/Clone | 10 μg | 5 μg |
| SfiI (40 U/μL) | 1.5 μL | 1 μL |
| 10× Buffer (Roche M) | 5 μL | 2.5 μL |
| Nuclease-free water | to 50 μL | to 25 μL |
| Total | 50 μL | 25 μL |
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