| Component | Amount |
| Expression Vector | 5 μg |
| SfiI (20 U/μL) | 1 μL |
| 10× Buffer (NEB 2) | 2.5 μL |
| 10× BSA | 2.5 μL |
| Nuclease-free water | to 25 μL |
| Total | 25 μL |
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