Component	Library Ligation	Library Control	Clone Ligation	Clone Control
Expression Vector	1 μg	1 μg	100 ng	100 ng
Library/Clone Insert	750 ng	-------	50 ng	-------
5× T4 DNA Ligase Buffer	20 μL	20 μL	4 μL	4 μL
T4 DNA Ligase (1 U/μL)	10 μL	10 μL	1 μL	1 μL
Nuclease-free water	to 100 μL	to 100 μL	to 20 μL	to 20 μL
Total	100 μL	100 μL	20 μL	20 μL