| Component | Amount |
| pSCV-1ZFGNN | 100 ng |
| SfiI (20 U/μL) | 0.5 μL |
| 10× Buffer (NEB 2) | 2 μL |
| 10× BSA | 2 μL |
| Nuclease-free water | to 20 μL |
| Total | 20 μL |
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