Component	Ligation	Control
1ZF GNN vector	100 ng	100 ng
1ZF GNN insert	50 ng	-------
5× T4 DNA Ligase Buffer	4 μL	4 μL
T4 DNA Ligase	1 μL	1 μL
Nuclease-free water	to 20 μL	to 20 μL
Total	20 μL	20 μL