| Component | Amount |
| pSCV-2ZFGNN | 5 μg |
| AgeI (5 U/μL) | 1 μL |
| SpeI (10 U/μL) | 1 μL |
| 10× Buffer (NEB 4) | 2.5 μL |
| 10× BSA | 2.5 μL |
| Nuclease-free water | to 25 μL |
| Total | 25 μL |
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