Component	Ligation	Control
2ZF GNN vector	200 ng	200 ng
1ZF GNN insert	100 ng	-------
5× T4 DNA Ligase Buffer	8 μL	8 μL
T4 DNA Ligase	2 μL	2 μL
Nuclease-free water	to 40 μL	to 40 μL
Total	40 μL	40 μL