Fig. 5.

Real-time RT-PCR analyses of gpr1, gpr2, gpr3 and gpr4 mRNA levels upon cultivation of T. atroviride on different carbon sources using act1 as a reference gene (a) and during in vitro biocontrol using sar1 as reference gene (b). The samples from cultures with glucose, glycerol and N-acetyl-glucosamine as sole carbon sources were harvested 12 h after transfer, those from cultures with chitin as sole carbon source or from cultures grown without carbon source were harvested 24 h after transfer. For monitoring gpr expression during in vitro biocontrol, T. atroviride P1 was either grown alone, or confronted with itself, or confronted with R. solani as host fungus. To allow comparison of mRNA levels of the four GPCR-encoding genes, mRNA levels of gpr1 on glucose (a) or PDA (b) were arbitrarily assigned the factor 1. The transcription ratio is presented in a logarithmic scale