Table 1.
Oligonucleotides used in this study
| Gene | Forward primera (5′ to 3′) | Reverse primer (5′ to 3′) | Product (bp) |
|---|---|---|---|
| (a) Degenerate primer pairs used to amplify fragments of reference genes | |||
| act1 | GTIGCICCIGARGARCAYCCI | ACRTCIACRTCRCAYTTCAT | 548 |
| gpd1 | GTIAARATHGTIAAYGAYAAYTT | GCIACIACISWITRYTCIGGYTT | 873 |
| tef1 | GTCCTCAGTCTTGTCATTTTTTTTCCT | GGAGGGGCCTGTCTGTGGGAC | 906 |
| sar1 | CTCGACAAYGCCGGAAAGAC | TTGCCAAGGATGACAAAGGGG | 833 |
| 28S | GGAACCTTTCCCCACTTC | AGTACCCGCTGAACTTAA | 1,461 |
| (b) Primer pairs used for quantification of transcripts by real-time PCR | |||
| gpr1 | TTGATCCAGACCTTCATGCCAGC | CATAAAAGGCCGCGACACGAA | |
| gpr2 | CTGCCCTCGTGTACATCTTCC | GCCTTCAGATGAGTGAAAGTCG | |
| gpr3 | GCCAGCATTGGAACCATCATCG | CCCAACACCAGATCGTAGCTCCA | |
| gpr4 | ATTCTAACTGGAGCCTGGTC | GTACTTTGTGGCTCTGGTTG | |
| act1 | GCACGGAATCGCTCGTTG | TTCTCCACCCCGCCAAGC | |
| gpd1 | CGTCGTAGTCCTTGTGGTTGACACC | TCCTCCCACGGTCTCTTCAAGG | |
| sar1 | CTCGACAATGCCGGAAAGACCA | TTGCCAAGGATGACAAAGGGG | |
| tef1 | TACTTCCCAGGCTGACTGCGCTAT | GGAGGGGCCTGTCTGTGGGAC | |
| 28S | TTTGAGTAAGAGCATACGGGGCC | GTTGATACATTCGAATGCCCACGT | |
a
R = A or G, Y = C or T, I = inosine (panel A)