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. 2010 Apr 16;5(4):e10190. doi: 10.1371/journal.pone.0010190

Figure 1. Overexpression of the OsNAS1 gene in rice endosperm.

Figure 1

(A) Schematic representation of the T-DNA in rice transformation vector. GluB-1, 2.3 kilo-base-pair rice glutelin B1 promoter; NAS1, coding sequence of rice OsNAS1 gene; Tnos, nopaline synthase terminator; 2×35S, double CaMV 35S promoter; TEV, tobacco etch virus 5′ untranslated region; bar: phosphinothricin acetyltransferase gene; Tvsp: soybean vegetative storage protein terminator; LB and RB, left and right T-DNA borders, respectively. (B) Reverse transcriptase PCR of OsNAS1 in EN1-EN4 transgenic and WT immature grains. EN1-EN4 are four transgenic lines overexpressing OsNAS1 in endosperm. OsACTIN was used as the internal standard. Total RNAs were extracted from the immature seeds 18 days after pollination. (C) Thirty five day-old seedlings of EN1, EN2, and WT grown in nutrient solutions, bar = 2 cm.