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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Gastroenterology. 2009 Oct 8;137(6):2030–2040.e5. doi: 10.1053/j.gastro.2009.07.078

Figure 2.

Figure 2

Elevated PTPN2 activity parallels decreased STAT phosphorylation in IFNγ-treated T84 cells. (a) PTPN2 was immunoprecipitated from whole cell lysates. Blots were probed for PTPN2 to show equivalent protein loading. The graph demonstrates IFNγ-stimulated PTPN2 activity (n=4). (b) Representative Western blots show phosphorylated cytoplasmic (Tyr701, above) and total STAT1 (below) after IFNγ treatment, followed by densitometric analysis (n=5). (c) Western blots and densitometry showing phosphorylated nuclear (above) and total STAT1 (below) in IFNγ-treated cells (n=5). (d) Phosphorylated cytoplasmic (Tyr705) and total STAT3 in IFNγ-treated T84 cells shown by representative Western blots and densitometry (n=4). Data in (b), (c) and (d) are shown as a percentage of the respective control. Asterisks indicate significant differences compared to the respective control (*=p<0.05, **=p<0.01, ***=p<0.001). #=p<0.05; ###=p<0.001 vs. 24 h IFNγ treatment.