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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Gastroenterology. 2009 Oct 8;137(6):2030–2040.e5. doi: 10.1053/j.gastro.2009.07.078

Figure 3.

Figure 3

PTPN2 knock-down enhances IFNγ-stimulated STAT1+3 phosphorylation. T84 cells were transfected with either non-specific or PTPN2 siRNA and treated with IFNγ (24h). (a) Representative Western blots for PTPN2 and lamin A/C followed by densitometric analysis (n=3). (b,c) Representative Western blots and densitometry showing STAT1+3 phosphorylation and expression (n=3). Figures (a) to (c) represent analyses of whole cell lysates. Asterisks indicate significant differences vs. the respective control (*=p<0.05, **=p<0.01, ***=p<0.001). ###=p<0.001 vs. 24 h IFNγ treatment of control siRNA-transfected cells. (d) Representative Western blots showing PTPN2 and lamin A/C, STAT1+3 expression and phosphorylation, in cytoplasmic and nuclear lysates (n=2).