Table 1. Oligonucleotides used for PAX6 amplification.

Exon	Sequences (5′-3′)	Annealing temperature (°C)
4	F:TGATGCAGCTGCCCGAGGATTA	68
	R:GGGGCGAGAGGGGGTGTGAGTTA
5	F:TTCCCCTTCCTCCTCTCCTTTCT	62.4
	R:GGGGGTCCATAATTAGCATCGTT
6	F:GCAAGGTCAGCACAAAAATAAAT	66.5
	R:TGCCCTGGGTCTGATGGA
7	F:AAAACGTAAGCTTGTCATTGT	58
	R:GAGAGGGTGGGAGGAGGTA
8	F:TTCCATGCCCAAAGTGATAG	60
	R:TTCCCAGGCCAACAAAAT
9	F:AAAATGGTGGTCAGGTAACTAAC	62.4
	R:TGAAAAGATGCCCAGAGAAATAA
10	F:TTGGTTGGAGGTAATGGGAGTG	62.4
	R:GGAAATCAGGTGGGACAGGTTAG
11	F:CGTGGGGAGGGCAGCAGTG	68
	R:ATGGAGCCAGATGTGAAGGAGGAA
12	F:GAAGGGCCAAATGGAGAAGAGA	68
	R:TGCAGACACAGCCAATGAGGT
13	F:GACTAGCTCGAGGCCCAATCTTA	64.8
	R:TTCCCTTTTCAATCCCCATCC
14	F:TTGCTGGAAATAAAAAGTGA	58.9
	R:CGGCTCTAACAGCCATTTTT
H-SSCP analysis of exon 5.	F:TTCTCCCTCATCTTCCTCTTCCTT	64.8
	R:GCAGTGGCCGCCCGTTGAC

A sequence of oligonucleotides and annealing temperatures was used for exon-by-exon PAX6 amplification. In the table, “F” indicates the forward strand and “R” indicates the reverse strand. Sequences are given in the 5′-3′ direction.