Figure 4.

Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for chondroition synthase synthesizing enzymes using RNA isolated from frontal cortex of non-transgenic (n = 6) and APPsw mice (n = 8). Threshold cycle number (2^−Ct) for the gene of interest was divided by the threshold for the GAPDH gene (2^−Ct) for each sample. There were no differences in expression for any of the five transcripts. Csgalnact1, Csgalnact2 (chondroitin sulfate N-acetyl-galactosaminyltransferase-1 and -2); Chsy1, Chsy2 (chondroitin sulfate synathase-1 and -2); Chpf (chon-droitin polymerizing factor).