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Steady state affinity model binding curves. The apparent dissociation (equilibrium) constants (Kd), calculated using a steady-state affinity model. A) Wild-type srfA DNA sequence with an average Kd equal to 9.59 nM. B) Consensus RE1-RE3 sequence with an average Kd equal to 11.27 nM. C) Consensus RE3 sequence with an average Kd equal to 4.94 nM.
