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Physical-chemical characterization of LFN14-263 and analogues. Analytical LC of the purified starting materials (a) and purified reaction product (b). The effluent was monitored at λ = 214 nm. c) SDS-PAGE analysis of the ligation reaction. The gel was stained with Coomassie Brillliant Blue. d-f) ESI-QTOF MS data for the purified, folded, and alkylated semisynthetic LFN analogues. The analytical details are reported in the experimental section.
