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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2010 Jan 7;23(2):201–209. doi: 10.1111/j.1755-148X.2010.00668.x

Figure 3.

Figure 3

αB-crystallin expression is regulated by both B-RAF signaling and proteasomal degradation. (A) qRT-PCR analysis of αB-crystallin mRNA in U0126 (16 hours) or control (DMSO) treated A375, SK-MEL-24, SK-MEL-5 cells. The graph represents the mean and s.d. for the percentage change in αB-crystallin mRNA level relative to actin from three independent experiments. (B) A375, SK-MEL-24, and SK-MEL-5 cells were treated with 10 μM U0126 for 16 hours followed by 10 μM MG132 treatment for 6 hours. Cell lystates were analyzed by Western blotting for αB-crystallin, pERK1/2 and ERK1/2 (loading control).