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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Cancer Res. 2010 Apr 13;70(8):3249–3258. doi: 10.1158/0008-5472.CAN-09-4009

Fig. 5.

Fig. 5

AEG-1 augments translation initiation and decreases degradation of MDR-1. A. The expression of the indicated proteins were detected in Hep-pc-4 (pc-4) cells and Hep-AEG-1-14 (AEG-1-14) cells treated or untreated with 10 µM LY294002 for 24 h by Western blot analysis. B. Hep-pc-4 (pc-4) and Hep-AEG-1-14 (AEG-1-14) cells were treated with MG132 (5 µM) for 24 h and the cell lysates were subjected to immunoprecipitation by anti-ubiquitin antibody (Ubi). The immunoprecipitates were subjected to Western blot analysis using anti-MDR1 antibody. IgG: normal mouse IgG. The lines in the figure indicate ubiquitinated MDR1. C. Hep-pc-4 (pc-4) and Hep-AEG-1-14 (AEG-1-14) cells were treated with MG132 (5 µM) for 24 h and the expression of MDR1 and β-tubulin was analyzed by Western blot.