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. 2009 Nov 26;13(8b):2408–2423. doi: 10.1111/j.1582-4934.2008.00675.x

Figure 3.

Figure 3

Distribution of GAD‐67+ interneurons in the dentate hilus and granule cell layer (DH and GCL) of a naïve adult hippocampus (A1), a hippocampus of an adult rat with kainic acid (KA)‐induced injury (B1), a naïve aged hippocampus (C1) and a hippocampus of an aged rat with KA‐induced injury (D1). A2, B2, C2 and D2 are magnified views of regions from A1, B1, C1 and D1 showing the morphology of GAD‐67+ interneurons. Scale bar, A1, B1, C1, D1 = 100 μm; A2, B2, C2, D2, = 50 μm. The bar charts (E1 and F1) compare numbers of GAD‐67+ interneurons in the DH + GCL (E1) and the dentate molecular layer (F1) between different groups. Note that, in the hippocampus of adult rats with KA‐induced injury, numbers of GAD‐67+ interneurons exhibit considerable (56%–62%) decline in different regions of the dentate gyrus, in comparison with numbers in similar regions of the age‐matched intact rats (P < 0.01 to P < 0.001). In contrast, numbers of GAD‐67+ interneurons in the dentate gyrus are mostly comparable between hippocampus of naïve aged rats and the hippocampus of aged rats with KA‐induced injury (P > 0.05).