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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Cancer Res. 2010 Mar 30;70(8):3259–3268. doi: 10.1158/0008-5472.CAN-09-4230

Figure 4. Noscapine inhibits TNF-dependent NF-κB activation.

Figure 4

(A) Effect of noscapine dose. KBM-5 cells were preincubated with indicated concentrations of noscapine for 12 h, treated with 0.1 nM TNF for 30 min, and subjected to EMSA to test for NF-κB activation. (B) Effect of noscapine exposure time. Cells were preincubated with 50 μM noscapine for the indicated times, treated with 0.1 nM TNF for 30 min, and then subjected to EMSA to test for NF-κB activation. Inhibition of NF-κB activation by noscapine is not cell-type specific. To determine the effect of noscapine on inducible NF-κB activation (C), A293 and H1299 cells were incubated with 50 μM noscapine for 12 h and then incubated with 0.1 nM TNF for 30 min. To determine the effect of noscapine on constitutive NF-κB activation (D), U266 and Du145 cells were incubated with indicated concentrations of noscapine for 12 h. Nuclear extracts were then prepared and assayed for NF-κB activation by EMSA.