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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Cancer Res. 2010 Mar 30;70(8):3259–3268. doi: 10.1158/0008-5472.CAN-09-4230

Figure 6. Noscapine represses NF-κB-dependent reporter gene expression induced by TNF and various plasmids.

Figure 6

(A) Noscapine inhibits the NF-κB-dependent reporter gene expression induced by TNF. A293 cells were transiently transfected with a NF-κB-expressing plasmid for 24 h. After transfection, cells were incubated with noscapine for 12 h and then treated with 1 nM TNF for an additional 24 h. The supernatants of the culture media were assayed for SEAP activity. (B) Noscapine inhibits the NF-κB-dependent reporter gene expression induced by TNF, TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1, IKKβ, and p65. Cells were transiently transfected with a NF-κB-expressing plasmid alone or with plasmids expressing the indicated proteins. After transfection, cells were incubated with 25 μM noscapine for 12 h and then incubated with the relevant plasmid for additional 24 h. TNF-treated cells were incubated with 25 μM noscapine for 12 h and then treated with 1 nM TNF for an additional 24 h. The supernatants of the culture media were assayed for SEAP activity. (C) Noscapine inhibits the COX-2 promoter activity induced by TNF. Cells were transiently transfected with a plasmid expressing a luciferase reporter gene under control of the COX-2 promoter for 24 h and then treated with noscapine for 12 h. Cells were then treated with 1 nM TNF for an additional 24 h, lysed, and subjected to a luciferase assay. Data in panels A-C are shown as fold activity over the activity of the vector control with the mean plus or minus the SD of the results of at least 3 independent experiments.