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. Author manuscript; available in PMC: 2011 Apr 16.
Published in final edited form as: Circ Res. 2010 Feb 25;106(7):1253–1264. doi: 10.1161/CIRCRESAHA.109.213116

Figure 5.

Figure 5

Subcellular localization of Nox4 in cardiac myocytes. A) Cultured cardiac myocytes were transduced with adenovirus harboring shRNA-scramble or shRNA-Nox4. Myocytes were co-stained with anti-Nox4 antibody and anti-F0F1 ATP synthase antibody, a marker of mitochondria. Merged images are shown on the right. B) Cultured cardiac myocytes were co-stained with anti-Nox4 and anti-p22phox antibodies. A merged image is shown on the right. C) Cultured cardiac myocytes were transduced with an expression plasmid harboring full length (FL) Nox4-HA or Nox4 lacking MLS (ΔN(75–578))-HA. Forty-eight hours after transfection, cells were stained with anti-HA antibody and DAPI. Truncation of the N-terminal region (1–74) causes disappearance of the perinuclear staining of Nox4 in cardiac myocytes. D) Cardiac myocytes were transduced with expression plasmids harboring either GFP alone or Nox4 (1–74)-GFP. Representative images of GFP, Mitotracker and merged images are shown.