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. 2010 May;130(1):23–33. doi: 10.1111/j.1365-2567.2009.03172.x

Figure 4.

Figure 4

Correlation between ex vivo and expanded human immunodeficiency virus (HIV) Gag-specific interferon-γ (IFN-γ) enzyme-linked immunosorbent spot-forming cell assay responses in blood from chronically HIV-infected subjects. (a) Peripheral blood mononuclear cells (PBMCs) from four HIV-infected women were assessed for HIV-specific IFN-γ production ex vivo (top panel) and following in vitro expansion with anti-CD3 monoclonal antibody for 28 days (bottom panel). (b) Correlation between IFN-γ responses detected in PBMCs ex vivo and after 28 days expansion. HIV-1 subtype C Gag overlapping peptides were divided into five pools and responses in these donors to each Gag pool were assessed. Net IFN-γ response to Gag was calculated by subtracting background IFN-γ production in each individual from Gag-specific responses. Each dot (b) represents an individual donor’s matched IFN-γ response to each of the five Gag pools in blood ex vivo and following expansion (day 28). Spearman Rank Test was used to test the correlation and a P-value <0·05 were considered significant.