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. 2010 May;130(1):74–82. doi: 10.1111/j.1365-2567.2009.03214.x

Figure 2.

Figure 2

Interleukin (IL)-4-induced CCL26 messenger RNA (mRNA) expression in monocytic cells is concentration-dependent and time-dependent. (a) U937 cells and (c) monocyte-derived macrophages (MDMs) were cultured for 24 hr in medium alone or in medium containing increasing amounts of IL-4 (0·1–50 ng/ml). (b) U937 cells and (d) MDMs were cultured in medium alone, or in medium containing 10 ng/ml of IL-4, for 1–72 hr. RNA was extracted from the cells using TRIzol. The expression of mRNA for CCL26 was evaluated by real-time polymerase chain reaction (PCR) and the data were normalized using 18S ribosomal RNA (rRNA) expression. Values for cytokine-stimulated cells were compared with those of control cells and data were then expressed as the mean ± standard error of the mean (SEM) of −delta delta Ct (−ddCt) from at least four independent experiments. **P<0·01 indicates statistical significance compared with the control; and ns is not significant.