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. 2010 May;130(1):74–82. doi: 10.1111/j.1365-2567.2009.03214.x

Figure 3.

Figure 3

Interleukin (IL)-4 induces CCL26 protein release from U937 cells and monocyte-derived macrophages (MDMs). U937 cells were cultured (a) for 24 or 48 hr in the absence or presence of 0·1–50 ng/ml of IL-4 or (b) for 24–72 hr in the presence of 10 ng/ml of IL-4. (c) MDMs were cultured for 24 or 48 hr in the absence or presence of 10 ng/ml of IL-4. Supernatants were collected and the levels of CCL26 protein were measured using enzyme-linked immunosorbent assay (ELISA). (a and b) Data are expressed as mean ± standard error of the mean (SEM) of at least three independent experiments. (c) Data are expressed as the mean of eight independent experiments. **P<0·01 and ***P < 0·001 indicates statistical significance compared with the control; † indicates statistical significance as compared to cells stimulated with the same concentration of IL-4 for 24 hr; and ns is not significant. Con, control.