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. 2010 May;130(1):74–82. doi: 10.1111/j.1365-2567.2009.03214.x

Figure 5.

Figure 5

Treatment with interferon-γ (IFN-γ) prevents subsequent interleukin (IL)-4-induced CCL26 messenger RNA (mRNA) and protein expression in U937 cells. U937 cells were cultured for 48 hr in medium alone (0) or in medium containing 0·1–100 ng/ml of IFN-γ. The U937 cells were then washed and incubated with fresh serum-free medium, either alone or containing IL-4 (1 or 10 ng/ml), for 24 hr. (a) RNA was isolated from the cells using TRIzol. The expression of mRNAs for CCL26 and β-actin was evaluated using the reverse transcription–polymerase chain reaction (RT-PCR). PCR products were visualized by gel electrophoresis in the presence of ethidium bromide. Data are representative of the results obtained from at least three independent experiments. (b) Supernatants were collected and CCL26 protein was measured using enzyme-linked immunosorbent assay (ELISA). Data are expressed as mean ± standard error of the mean (SEM) of at least three independent experiments. ***P < 0·001 compared to the Control (0) with no IL-4; ns, not significant.