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. 2010 May;130(1):74–82. doi: 10.1111/j.1365-2567.2009.03214.x

Figure 6.

Figure 6

Treatment with interferon-γ (IFN-γ) decreases total signal transducer and activator of transcription 6 (STAT6) protein and subsequent interleukin (IL)-4-induced STAT6 phosphorylation in U937 cells. (a) U937 cells were cultured for 24 or 48 hr in medium alone (none) or in medium containing 100 ng/ml of IFN-γ. U937 cells were then washed and incubated with fresh serum-free medium alone or with medium containing IL-4 (10 ng/ml) or IL-4 and IFN-γ for 10 min (co-inc). Cell lysates were harvested in 2 × Laemelli sample buffer and analysed by Western blotting for phosphorylated STAT6 (P-STAT6), total STAT6 (T-STAT6) and β-actin. (a) The blots shown are representative of the results obtained in three independent experiments. (b) Densitometry was used to measure the levels of total STAT6 and β-actin, and the values were expressed as a percentage of the density levels of each respective protein present in U937 cells cultured for 48 hr in the presence of medium alone (none) ± standard error of the mean (SEM) from four independent experiments. **P < 0·01 compared to cells cultured for 24 hr in the presence of medium alone.