Figure 4.

Interleukin-17A (IL-17A) and IL-17A-associated cytokine transcripts were decreased in Toll-like receptor-6-deficient (TLR6^−/−) mice with experimental hypersensitivity pneumonitis (HP). Cyokine transcript analysis of whole lung samples was performed by Taqman. Transcript expression of IL-17A (a), IL-23 (b), IL-21 (c) and IL-6 (d), in whole lungs of wild-type (WT) and TLR6^−/− mice at 1, 4, 8 and 16 days after the last Saccharopolyspora rectivirgula antigen (SR-Ag) challenge was determined. Results are expressed as fold change over transcript expression in WT samples collected at day 1 after the last SR-Ag challenge. Data represent the mean ± SEM, with n = 4 or n = 5 for each group. One-way analysis of variance and Newman–Keuls multiple comparison test were used to analyse significance between groups. **P ≤ 0·01, ***P ≤ 0·001.